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KMID : 0878220010130010088
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Journal of Sasang Constitutional Medicine
2001 Volume.13 No. 1 p.88 ~ p.96
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Effects of Yuldahansotang water extract on Cultured Spinal Sensory Neurons Damaged by Xanthine Oxidase/Hypoxanthine
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Abstract
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To evaluate th effect of Yuldahansotang(YHT) water extract on cultured mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MTT assay, NR assay, Neurofilament evzymeimmuno assay and LDH activity assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the surival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay. 2. MTT50 value and NR50 value of XO/HX were 20 mU/㎖ XO/0.2mM HX and 40 mU/㎖ XO/0.2 mM HX.. 3. YHT water extract have efficacy of increasing neurofilament. 4. YHT water extract have efficacy of increasing LDH activity. From above the results, It is concluded that YTH has marked afficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.
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